Search for synthetic and natural substances with in vitro bioactivity against Mycobacterium tuberculosis.

Maria-Fernanda Moreno , Wellman Ribón

Affiliation: Universidad Industrial de Santander. Colombia.

DOI: 10.17160/josha.5.1.380

Languages: Spanish, Castilian

tuberculosis (TB) is still considered as an important worldwide health problem. For this reason, national and international health agencies encourage the researchers to improve and strengthen the control strategies and disease treatment. Currently, there have been important advances in the discovery and development of drugs that probably will be included in the standard scheme of anti-TB treatment, however for many of the current treatments, there have been resistant strains reported and even worst for most of the new treatments resistance is expected, making it necessary to maintain a continuous search for treatment alternatives.


Psychology based on evidence and panic disorder; analysis of a case study

Jorge Alberto Velásques Castrillón

DOI: 10.17160/josha.5.1.377

Languages: Spanish, Castilian

This single case study aims to analyse the case of a patient suffering panic disorder, accompanied by a recurrent depressive disorder. As a treatment, therapies based on empirical evidence were used. During the evaluation phase, a symptoms severity scale for panic disorder, a depression inventory from Beck, 1979 and a clinical interview were used; additionally, therapeutic targets were proposed, and panic cognitive therapy was applied; the therapy was developed during 17 treatment sessions, where the techniques of psychoeducation, cognitive restructuring, relaxation and interceptive exposure were used; At the end of the treatment there was a significant decrease in the symptoms of panic, anxiety and depression that helped to improve the overall conditions of the patient. When comparing the results obtained after the treatment process, the results show congruencies with the available literature.


Search for synthetic and natural substances with in vitro bioactivity against Mycobacterium tuberculosis. Part I

Maria-Fernanda Moreno , Wellman Ribón

Affiliation: Universidad Industrial de Santander. Colombia.

DOI: 10.17160/josha.4.6.367

Languages: Spanish, Castilian

INTRODUCTION: tuberculosis (TB) is still considered as an important worldwide health problem. For this reason, national and international health agencies encourage the researchers to improve and strengthen the control strategies and disease treatment. Currently, there have been important advances in the discovery and development of drugs that probably will be included in the standard scheme of anti-TB treatment, however for many of the current treatments, there have been resistant strains reported and even worst for most of the new treatments resistance is expected, making it necessary to maintain a continuous search for treatment alternatives. OBJECTIVE: determine the in vitro antimycobacterial activity of 15 synthetic molecules, 14 essential oils and 6 extracts from endemic plants growing in the department of Santander, Colombia.


Apply by December 15th for the NEW IMBS Double Master Program in Biomedical Sciences: MSc from the University of Buenos Aires and MSc from Freiburg University

Christoph Borner, Marta Mollerach, Cristina Arranz, Roland Mertelsmann

DOI: 10.17160/josha.4.6.361

Languages: English

The International Master of Science Biomedical Sciences (IMBS) is an international, interdisciplinary modular and fulltime study program for graduates with professional experience in health or life sciences, medicine or related fields. The IMBS program is a joint program between University of Freiburg (ALU) and the University of Buenos Aires (UBA) in Argentina. After successful graduation, the students will receive a double binational Master degree from the University of Buenos Aires and from Freiburg University. The program which is designed as an intense time of studies and research with practical training qualifies for success in academia or the private sector. It starts with 8 months basic unit in Buenos Aires, continues with an 8 months advanced unit in Freiburg including 5 months individually supervised lab rotation and ends with an 8 months Research Project which leads to a Master Thesis.


Die Tochter des Kaufmanns

Mitra Zarif-Kayvan

Languages: Persian

The fairy tale ''The daughter of the businessman'' is an Iranian folktale which has been delivered orally from one generation to another in various versions. This fairy tale was in fact made popular by the famous Iranian story-teller Fazlollah Mohtadi, known as ''Sobhi'' in ''Diwan Balkh''and from Samad Behranghi in ''the fairy tales from Azerbaijan'' and it has been delivered in various versions with different titles. The fairy tale revolves around a beautiful, clever, brave and confident girl who defends herself all alone against prejudice and abuse. She embarks on a long, dangerous and an adventurous journey. At the end, she manages to prove the truth and defend herself. Finally, she marries the innkeeper who values her beauty, intelligence and wisdom and builds a life with him filled with harmony, respect and love. In this book, the Author and the Illustrator portray this fairy tale in her own version in German and Persian languages.


Scientific cooperation between Italy and Israel: A perspective looking to the future

Mario Pagliaro

Affiliation: Istituto per lo Studio dei Materiali Nanostrutturati, Palermo, Italy

DOI: 10.17160/josha.4.2.294

Languages: English

From renewable energy through neuroscience, from chemistry to nanotechnology, the scientific cooperation between Italy and Israel touches many fields and involves hundreds of scholars and students in both countries. We identify the main research policy factors that led to this successful outcome, and those that might drive forthcoming cooperation between these two Mediterranean countries whose science linkages have a long and significant history.


Characterization of the AZF region of the Y chromosome in Native American haplogroup Q

Evguenia Alechine, Werner Schempp, Daniel Corach

Affiliation: University of Buenos Aires

DOI: 10.17160/josha.3.4.219

Languages: English

The Y chromosome is a genomic niche for genes involved in male gamete production. The existence of an azoospermia factor (AZF) in its long arm is a key genetic determinant for spermatogenesis since its deletion is associated with infertility. Deletions in the AZFc region are the most frequent known genetic cause of male infertility. This region contains eight gene families involved in spermatogenesis, including Deleted in Azoospermia (DAZ) and the Chromodomain Protein Y (CDY) genes. AZFc displays significant variation across the male population; nonetheless, the phenotypical consequences of some of these variants remain unclear. Many Y-chromosome geographically differentiated haplogroups have been defined in the human population. However, the information available on the Y chromosome sequence in GenBank belongs only to the European haplogroup R. Recent studies have shown that high mutation rates have driven extensive structural polymorphism among human Y chromosomes.


Assembly and disassembly of Rad51 filaments on single-stranded DNA: A novel assay to study the dynamics of protein-ssDNA interactions at the single-molecule level

Mariella Franker

DOI: 10.17160/josha.3.3.198

Languages: English

Eukaryotic recombinase protein Rad51 is the key player in homologous recombination, an essential DNA repair mechanism used for the repair of double-strand breaks. Double-strand breaks can lead to chromosome fragmentation and are particularly hazardous during and shortly after DNA replication. The mechanism of homologous recombination is highly conserved between species and recombinase proteins are expressed in a wide range of prokaryotic and eukaryotic cells. The primary event in homologous recombination is the formation of a helical nucleoprotein filament on single- stranded DNA overhangs around double-strand breaks. The nucleoprotein filament mediates all subsequent steps of homologous recombination and is capable of performing strand exchange reactions unassisted in vitro. Dynamic assembly and disassembly interactions between the nucleoprotein filament and its DNA substrate are essential for strand exchange.


Homologous recombination: Single-molecule experiments and their lessons for the in vivo situation

Mariella Franker

DOI: 10.17160/josha.3.3.197

Languages: English

Homologous recombination is an essential DNA repair mechanism in both prokaryotes and eukaryotes. It especially plays an important role in the repair of broken or stalled replication forks and is vital for proper chromosome segregation and immunoglobulin diversity. The main event in homologous recombination is the formation of a nucleoprotein filament by RecA-like proteins. Assembly of this filament is the rate-limiting step in recombination and it mediates subsequent stages of repair. Single-molecule experiments have given great insights into the physical mechanism and function of the nucleoprotein filament. In vivo, however, many recombination mediators are involved in the processes and various complex pathways are activated. INSTITUTION: VU University Amsterdam, Faculty of Sciences, Department of Physics and Astronomy, Physics of Living Systems


Bioinformatic Studies on Buffalo Prolactin Derived Anti-Angiogenic Peptide

Pulak P. Kumar, Pratishtha Singh

Affiliation: Student

DOI: 10.17160/josha.3.3.193

Languages: English

A 14-amino acid sequence within the buffalo prolactin (buPRL) protein has been identified by BLAST search as similar to that of somatostatin, the gold standard for determining anti-angiogenic activity. A synthetic peptide with the same sequence has been shown to exhibit powerful anti-angiogenic activity, possibly by functioning as a kallikrein-kinin system (KKS) antagonist. In order to further study this peptide’s anti-angiogenic nature, bioinformatics tools were used to analyze its interaction with the bradykinin B1 receptor, which is a component of the KKS. Molecular docking studies were conducted in silico using structures of bradykinin B1 receptor obtained by homology modeling using SWISS-MODEL via the EXPASY web server, as well as a structure of the synthetic peptide that was modeled by the PEP-FOLD de novo modeling server.