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Optimization of long-term Breast Cancer Cell Culture using live Imaging in an automated Perfusion System
DOI: 10.17160/josha.7.5.706
A significant quantity of questions remains in cellular biology. Some of them cannot be answered by applying traditional analytical techniques and request the development of new tools for research. Nowadays, the integration of microdevices and analytical platforms enables the acquisition of biological data in an agile and low-cost manner. Additionally, perfusion technologies allow us to recreate physiological microenvironments. For this reason, the integration of live-cell microscopy, perfusion, and microsystems, will enable control of the delivery of soluble factors and the tracking of single cells. These platforms have a vast potential to result in a better understanding of cell behavior, generating evidence for potential predictive models for disease treatment. We have developed a robust live-imaging fluidic system for studying breast cancer cells. Our data suggest fast and short pulses as the preferred strategy for perfused assays.
Poverty is the Mother of Health
DOI: 10.17160/josha.7.4.705
People with little education, low income, and professional status are at two to three times greater risk of developing diabetes or cancer, of having a heart attack, or a stroke. For them, these diseases appear comparatively earlier, are more severe, and have more serious consequences for everyday life. Franziska Buttgereit is a mezzo-soprano who has been a member of the Freiburg Theatre's extra choir since 2010 and its first director since 2017. In 2015 she began her singing studies with Prof. Christiane Libor at the Musikhochschule Schloss Gottesaue in Karlsruhe and has been collaborating with the University of Music and the Stadttheater Freiburg, which has led to a small role in performances and the DVD production of the same name "Cendrillon" by Jules Massenet.
JOSHA SCHOLARSHIP FUND to support young students, artists, scientists from all around the world
DOI: 10.17160/josha.7.4.703
JOSHA JOURNAL/ IASHA ACADEMY IASHA e.V. has provided scholarships to students since 2015. The requests for scholarships have significantly increased during the Corona Pandemic which is why we need your help to rapidly support students, artists and scientists who are in financial or economic distress. Scholarships are made available to local and international students to cover for their expenses. By reducing their financial barriers, we can help make education and career goals easier to obtain. In addition to the financial aid, our open-access internet journal JOSHA (www.josha-journal.org) lays out a forum where students, artists, and scientists can present their achievements to the public. There, you can value their presentations and follow-up on the impact of your funding. With your donation, you set a sign of philanthropy and help to continue our mission to support the future careers of many young and gifted people. Thank you for your donation! http://josha-journal.
Pandemic SARS CoV -2 - a Romanian perspective. The perception of a septuagenarian doctor
DOI: 10.17160/josha.7.4.702
The SARS-CoV-2 pandemic reveals everywhere in the world, and of course also in Romania, individuals’ ability to respond to danger, but also their degree of compliance with the medical, social and political regulations in order to act responsibly. Behavioral diversity, level of education, media influence have left their mark on the response of society to the spread of the infection to such an extent that the number of cases has resumed its daily growth. The perspective of the pandemic evolution remains unclear, and Romania, despite the medical effort made, does not seem to have had the complete revelation of the danger yet. Humanity lost in self-admiration does not yet realize the mortal danger of this virus.
COVID-19 Impacts on High Education: Virtual Learning Challenges on University of Prishtina
DOI: 10.17160/josha.7.4.700
The cease of the high educational institutions in Kosova, as a result of the preventive measures against the spread of Covid-19 has a high causatum on the traditional system of academia, moreover, as we evidence later the global preventive measures has affected the all education systems in the world. Hence, there was a new kind of feeling, with new immediate responsive state caused by the change of learning patterns. While this eminent experience of shifting from traditional learning to virtual learning domain has a result with immediate quarantine in Kosovo, and a new style of living, a new experience of education, and a new teaching interaction with students, especially in the faculty of the architecture, University of Prishtina. The comments presented in this paper investigated the COVID-19 phenomenon in the University of Prishtina, from the pedagogue point of view, where to many learning habits and teaching methods are being radically changed in front of new contagious diseases.
An open book
DOI: 10.17160/josha.7.5.699
In the 1990s, scientists started to discuss how to sequence the human genome, the complete list of genes encoded in human DNA. Two research groups, one directed by James Watson and funded by NIH grants, and another one supervised by Craig Venter and financed by private funding, started a race to be the first one to obtain the complete sequence. In 2000, the US President Bill Clinton, together with Venter and Francis Collins (Watson’s successor), announced that the first draft of the human genome was available. In the last years, cost reduction for sequencing the whole human DNA open several research fields, such as cancer genomics, pharmacogenomics, diagnosis of rare diseases, and genomics of pregnancy, among many others. In the present article, originally published in El Gato y La Caja, the history and impact of this discovery is presented.
Mapmaking and Storytelling
DOI: 10.17160/josha.7.4.698
In this thesis, I analyse artists’ books and fanzines which were created for the exhibition ‘Subjective Maps / Disappearances’ in 2013 at the National Gallery of Iceland. The event was organized as part of the artistic research undertaken within Little Constellation, a contemporary art network with a focus on 17 geo-cultural micro-areas and small states of Europe such as San Marino, Iceland, Monaco, Gibraltar, and Cyprus. These areas and their communities are mostly understudied, systematically neglected in comparative analyses, and often only known through "uncertain" stereotypes such as tax havens, smugglers, or duty-free shopping centers. For the exhibition, 37 artists born or based in these areas exhibited artists’ books and fanzines that will become part of the permanent public collection of the San Marino National Gallery. A selection of the works was analyzed through a rhythmic analysis, taken from Henri Lefebvre, allowing the examination of time and space together.
Editorial Volume 7, Issue 3
DOI: 10.17160/josha.7.4.693
Dear JOSHA readers, once again we find ourselves saying goodbye and closing another wonderful issue! This bi-monthly issue is full of wonderful articles, including two of the candidates and possible winners of the 2020 Demetrios Award. In addition, we are re-packaging this section with new information that is useful to you and important to us, such as the new button to make your donation to the journal. On the other hand, don't miss the opportunity to visit our social media and get to know us from the Instagram gallery and the Facebook wall. Remember that you can comment on the articles and also provide your star ranking.
COVID-19 in Kosovo: Point from an Urbanite
DOI: 10.17160/josha.7.3.685
In the continuum of evolution and adaptation of living organisms, ranging from microorganisms to human beings, there are certain cycles of time that carry with its cardinal changes that human beings must face and find the possible public health solutions. The new millennium brings with it a new public health, and public order issues, thus, manifestation completely a new urban lifestyle. Therefore, we have come into a situation where a number of questions need to be raised about the necessity of redefining many daily life habits. The comments presented in this paper investigated the COVID-19 phenomenon in Kosovo, from urbanites point of view, where to many urban habits and lifestyle are being radically changed in the front of new contagious disease. Focusing on the urban lifestyle, public health, public order, high education, and possible urban depressions related to the quarantine conditions.
Perfect Pitch
DOI: 10.17160/josha.7.3.678
Mozart had perfect pitch, as did Beethoven, Bach, and Jimi Hendrix. However, this ability does not a gifted musician make, as studies continually show that prodigies and amateurs alike may are capable of it. Franziska Buttgereit is a mezzo-soprano who has been a member of the Freiburg Theatre's extra choir since 2010 and its first director since 2017. In 2015 she began her singing studies with Prof. Christiane Libor at the Musikhochschule Schloss Gottesaue in Karlsruhe and has been collaborating with the University of Music and the Stadttheater Freiburg, which has led to a small role in performances and the DVD production of the same name "Cendrillon" by Jules Massenet.