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Growth hormone (GH) modulation of Epidermal growth factor (EGF) signalling in human mammary epithelial cells
Verena Wölker
Affiliation: Uniklinik Freiburg
Keywords: Breast cancer, growth hormone, epidermal growth factor, ERK, AKT
Categories: Medicine
DOI: 10.17160/josha.2.3.36
Languages: English
This study demonstrates crosstalk between Growth Hormone (GH) and Epidermal Growth Factor (EGF) in two different breast cancer cell lines. Long-term modification of cell viability and the ERK1/2 and AKT pathways was only detected in MDA-231 cells. By simultaneous treatment with GH and EGF, the interaction between GH and EGF in MCF-7 cells could be transiently abrogated by GH-pre-treatment. Co-treatment with GH and EGF diminished cell viability in MCF-7 and MDA-231 cells. Furthermore, a direct influence between GH and EGF signaling resulted in less ERK phosphorylation in MCF-7 cells, whereas in MDA-231 cells less AKT phosphorylation could be observed. EGFR-down regulation due to GH is a potential reason but keeps to be elucidated.